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Gary Schroth, PhD provides an introduction to index hopping, describes how to reduce index hopping degrees by subsequent library prep greatest methods, and discusses how to remove index-hopped reads making use of exclusive dual indexing combos.
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At Illumina, our aim is to apply modern technologies on the Evaluation of genetic variation and performance, creating scientific tests possible that were not even conceivable just some several years ago. It truly is mission critical for us to provide ground breaking, versatile, and scalable solutions to meet the requires of our consumers.
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Make entire-genome sequencing libraries and efficiently interrogate samples with confined readily available DNA.
Set C Index Adapters Consult with Index two (i5) Orientation For more info regarding how to enter website i5 bases around the sample sheet in forward or reverse complement orientation.
Established D Index Adapters Confer with Index 2 (i5) Orientation For more info on how to enter i5 bases on the sample sheet in ahead or reverse complement orientation.
Index two: the i5 index column is in the reverse complement orientation (are definitely the reverse complement from the i5 indexes inside the sample sheet).
Explore how AI is reworking genomic analysis, driving scientific insights, and enabling multiomic discovery
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Library prep, enrichment, and probe panel reagents should be requested separately. Compatible library prep kits: